ABSTRACT
The immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.
O sistema imune de peixes teleósteos tem mecanismos responsáveis pela defesa contra bactérias e atua através de proteínas presentes em diversos tecidos. A ação destas proteínas pode ser avaliada pela atividade bactericida do soro, sendo esta uma importante ferramenta para analisar o sistema imune. O pacu, Piaractus mesopotamicus, é um peixe nativo muito importante para aquicultura nacional, entretanto há pouco conhecimento sobre o funcionamento de seu sistema imune. Assim foi realizado experimento para padronizar e avaliar a eficiência do ensaio de atividade bactericida. Resumidamente, peixes foram desafiados por Aeromonas hydrophila e amostradas uma semana após o desafio. A infecção bacteriana promoveu um aumento na concentração de proteínas protetoras, resultando em diminuição dos valores de unidades formadoras de colônias ou expressos também como aumento da atividade bactericida do soro. O protocolo se mostrou confiável, sendo apropriado para determinar a atividade bactericida do soro de pacu nas condições experimentais.
Subject(s)
Animals , Anti-Bacterial Agents , Immunity, Humoral/immunology , Immune System/physiology , Fishes/classificationABSTRACT
Columnaris disease is one of the main causes of mortality in tilapia rearing and is responsible for large economic losses worldwide. Hematology is a tool that makes it possible to study organisms' physiological responses to pathogens. It may assist in making diagnoses and prognoses on diseases in fish populations. The hematological variables of nile tilapia were studied in specimens with a clinical diagnosis of columnaris disease and in specimens that were disease-free. The total erythrocyte count, hemoglobin rate, hematocrit percentage, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), organic defense blood cell percentages (leukocytes and thrombocytes) and hepatosomatic and splenosomatic index were determined. The results showed that there were changes in the erythrocytic series and in organic defense blood cells, in the fish infected with the bacterium, with reductions in erythrocytic variables and significant increases in the numbers of circulating lymphocytes and neutrophils.